Setup BUSCO using a Docker Image in Ubuntu 16.04

Install Docker

Update repositories and add the docker repository to APT sources

sudo apt-get update
sudo apt-add-repository 'deb https://apt.dockerproject.org/repo ubuntu-xenial main'
sudo apt-key adv --keyserver hkp://p80.pool.sks-keyservers.net:80 --recv-keys 58118E89F3A912897C070ADBF76221572C52609D
sudo apt-get update

Be sure to install from Ubuntu 16.04 default repository.
```
apt-cache policy docker-engine
```
It should appears this output:

#Output of apt-cache policy docker-engine
docker-engine:
  Installed: (none)
  Candidate: 1.11.1-0~xenial
  Version table:
     1.11.1-0~xenial 500
        500 https://apt.dockerproject.org/repo ubuntu-xenial/main amd64 Packages
     1.11.0-0~xenial 500
        500 https://apt.dockerproject.org/repo ubuntu-xenial/main amd64 Packages

Install Docker and verify that daemon has started and process enabled

sudo apt-get install -y docker-engine
sudo systemctl status docker

The output should be:

Output
● docker.service - Docker Application Container Engine
   Loaded: loaded (/lib/systemd/system/docker.service; enabled; vendor preset: enabled)
   Active: active (running) since Sun 2016-05-01 06:53:52 CDT; 1 weeks 3 days ago
     Docs: https://docs.docker.com
 Main PID: 749 (docker)

Execute Docker.

a) With root privilegies You need root privilegies to execute docker; you need to use sudo command to execute:

sudo docker

b) If your user is in the docker group.

To add my user to docker group:

sudo usermod -aG docker $(whoami)

To add an specific user to docker group:

sudo usermod -aG docker username

Example:

sudo usermod -aG docker  ricardoparra

Note: It’s necessary logout and login again.

Using the docker command

docker
docker info

Try docker images
```
docker run hello-world
docker images
```

Download and execute BUSCO

Download (pull) the BUSCO docker image.
```
docker pull ezlabgva/busco:v4.0.4_cv1
```
Execute BUSCO with an specific user Before execute, the user should make the working directory (WD). In this example the WD is: /home/ricardoparra/busco_host_mount.

mkdir /home/ricardoparra/busco_host_mount

Execute docker specifying working directory and with genome.fna input file.

docker run -u $(id -u) -v /home/ricardoparra:/home/ricardoparra/busco_host_mount -w /home/ricardoparra/busco_host_mount ezlabgva/busco:v4.0.5_cv1 busco -i genome.fna

Source: https://www.digitalocean.com/community/tutorials/como-instalar-y-usar-docker-en-ubuntu-16-04-es